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This is especially genuine for immunohistochemistry where ten% NBF has actually been shown to get a bad fixative (Fig. two). Some problems with the use of 10% NBF as a fixative are actually corrected via the introduction of other experimental techniques which include antigen retrieval (Shi et al. 2001) and the use of smaller amplicons in RT-Q-PCR (

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The aforementioned actions of tissue processing is usually accomplished in both a handbook or an automatic trend. Handbook processing is time-consuming, Using the length of most manual protocols exceeding the size of a daily perform working day.When deciding on a clearing agent, it can be crucial to pick an agent that does not more than harden the

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